Micro-CT scan confirmed multiple osteolytic lesions at the femur, spine and skull. Two weeks after intracardiac injection, metastasis to the brain, spine and femur was detected with bioluminescence imaging with an increasing intensity by week 7. Metastasis was found in 62.3% of the animals. The findings of micro-CT, MRI and the histology were correlated with the 'hot' lesions seen on the bioluminescence imaging.
#SANDRA ORLOW NUDE PICTURE SERIAL#
After injection of the tumor cells, serial bioluminescence imaging was performed for 7 weeks. The animals underwent left ventricular injection of 2x105 MDA-MB-231Bo-Luc cells. Animal experiments were performed in 6-week-old female nude mice. The purpose of this study was to develop a nude mouse model of bone metastasis by performing intracardiac injection of breast cancer cells under ultrasonography guidance and we wanted to evaluate the development and the distribution of metastasis in vivo using micro-CT, MRI and bioluminescence imaging. Park, Young Jin Song, Eun Hye Kim, Seol Hwa Song, Ho Taek Suh, Jin Suck [Yonsei University College of Medicine, Seoul (Korea, Republic of) Choi, Sang Hyun [Korean Minjok Leadership Academy, Heongsung (Korea, Republic of) All rights reserved.Ī Bone Metastasis Nude Mouse Model Created by Ultrasound Guided Intracardiac Injection of Breast Cancer Cells: the Micro-CT, MRI and Bioluminescence Imaging AnalysisĮnergy Technology Data Exchange (ETDEWEB) The present study can be translated to the clinic by various effective methods of fluorescently labeling tumors. Surgical outcomes in orthotopic nude mouse models of human colon cancer were significantly improved with FGS. FGS resulted in a cure in 67% of mice (alive without evidence of tumor at >6 mo after surgery) compared with only 37% of mice that underwent BLS (P = 0.049). The median overall survival increased from 16 wk in the BLS group to 31 weeks in the FGS group. FGS resulted in decreased recurrence compared with BLS (33% versus 62%, P = 0.049) and lengthened disease-free median survival from 9 to >36 wk. All mice with primary tumor that had undergone FGS had complete resection compared with 58% of mice in the BLS group (P = 0.001). Pre- and post-operative images with the OV-100 Small Animal Imaging System (Olympus Corp, Tokyo Japan) were obtained to assess the extent of surgical resection. Tumors were resected under bright light surgery (BLS) or FGS. We established fluorescent orthotopic mouse models of human colon cancer expressing a fluorescent protein. We inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic nude mouse models of human colon cancer. Metildi, Cristina A Kaushal, Sharmeela Snyder, Cynthia S Hoffman, Robert M Bouvet, Michael (authors)įluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model. Conclusions: Anti-EGFR therapy enhances the radiosensitivity of human lung adenocarcinoma xenograft in nude mouse. Sensitizing enhancement ratio of combination group was 1.5 in growth and 1.7 in regrowth. Tumor regrowth delay time in combination therapy and irradiation alone groups was 23.4 and 10.2 days. Results: For A549 xenografts in radiation alone, gefitinib alone and combination therapy groups, the percentage of tumor growth inhibition was 22.7%, 12.4% and 38.2%, respectively (F=25.75, P=0.000). Percentage of tumor growth inhibition, growth delay time and regrowth delay time were evaluated. Tumor diameter was measured every other day. In the combination group, radiotherapy was performed 2 hours after Gefitinib administration.
Gefitinib was daily administered by gavage at 100 mg/(kg Radiation schedule was 3 fractions of 5 Gy, once daily. The mice were derided into 4 groups: control, irradiation alone, Gefinitib alone and radiation combined with Genifitib. Methods: Human lung adenocarcinoma cell line A549 was used to establish nude mouse xenograft tumor model. Objective: To investigate the effect of Gefitinib on radiosensitivity of human lung adenocarcinoma xenograft in nude mouse. Wang Hui Li Tianran Tian Jiahe Qu Baolin Zhu Hui International Nuclear Information System (INIS) The effect of .Īnti-EGFR therapy radiosensitizes human lung adenocarcinoma xenograft in nude mouse Xenogen in vivo imaging system was used to monitor tumor growth and metastasis. Methods: Withaferin A was injected through a portal vein to the orthotopic liver tumor in a nude mice model. Purpose: To investigate the effect of withaferin A on tumor growth and metastasis in liver in a nude mouse model. Withaferin A Suppresses Liver Tumor Growth in a Nude Mouse.
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